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GrainGenes Marker Report: GBM1274

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Locus
GBM1274
Type
Microsatellite
Chromosome
6H
Map
Barley, B73xCPI-6H109.4
[ Show all 6 ]
[ Show Nearby Loci ]
Map Data
Barley, B73xCPI
[ Show all 6 ]
Species
Hordeum vulgare
Probe
GBM1274
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceWenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
ReferenceVarshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remark
Number of populations contributing to the placement of GBM1274 in the Barley, Consensus 2006 DArT maps = 1
[ Show all 8 ]



Probe
GBM1274
Locus
GBM1274
GBM1274-6H
Reference
ReferenceHearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391.
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceVarshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks
Primer sequences for this SSR to be released in the public domain soon (noted: September 2006). Those interested should contact Andreas Graner
Clone HU04D08u
Type
SSR
EST
PCR primers
CGTCCACTCTTTTCTGAGGG
TGGGCTCAATTTTCCTGTTC
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
BQ663824
DNA Library
IPK-HU
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
Data Source
Graner, Andreas
Stein, Nils2006.10



Locus for Probe
GBM1274-6H
Type
Microsatellite
Map
Hv-Consensus2006-Stein-6H125.1
Hv-DxR-Stein-6H118.35
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Stein
Barley, OWB, Stein2006
Probe
GBM1274

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