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GrainGenes Marker Report: HVM2

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Locus
HVM2
Type
Microsatellite
Synonym
SynonymHVM002B-2
Chromosome
1H
Map
Barley-consensus-2003-1H69.1
[ Show Nearby Loci ]
Map Data
Barley consensus 2003
Probe
HVM2
Data Source
Langridge, Peter J.



Probe
HVM2
Locus
HVM2a
HVM2b
HVM002B-1
HVM002B-2
HVM2
Reference
ReferenceLiu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Remarks
Insert contains repeat (GA)11.
Type
PCR
SSR
PCR primers
5' CAGGTGTCTAGTGGGTGCCT 3'
5' TTACATACCAAGGAGCAATCCC 3'
SSR size
0.201
Amplification Conditions
The 'touchdown' PCR comprised 48 cycles of 94oC for 1 min denaturing and 72oC for 1 min extension. Annealing (30 s) temperatures were progressively decreased by 1oC every second cycle from 64oC to 55oC. Annealing conditions of 1 min at 55oC were maintained during the final 30 cycles. The reaction ended with a 5-min extension at 72oC.
Insert Enzyme
TaqI
Source Species
Hordeum vulgare
Source Germplasm
Old ZZ (barley)
DNA Origin
Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.
Clone Vector
pGEM-7zf(+)
Vector Enzyme
ClaI
Data Source
Saghai Maroof, M.A.97.01.02



Locus for Probe
HVM2a
Type
Microsatellite
Synonym
Correct nameHVM002B-1
Chromosome
5H
Chromosome Arm
5HS
Map
Hordeum-CxB-5H25.8
[ Show Nearby Loci ]
Map Data
Barley, Cali-sib x BowmanBC, QTL
Probe
HVM2
ReferenceLiu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.



Locus for Probe
HVM2b
Type
Microsatellite
Synonym
Correct nameHVM002B-2
Chromosome
7H
Chromosome Arm
7HS
Probe
HVM2
ReferenceLiu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.



Locus for Probe
HVM002B-1
Type
Microsatellite
Synonym
Other nameHVM2a
Map
Hordeum-Bins-5H58.4
[ Show Nearby Loci ]
Map Data
Barley, BinMap 2005
Probe
HVM2



Locus for Probe
HVM002B-2
Type
Microsatellite
Synonym
Other nameHVM2b
SynonymHVM2
Chromosome
7H
Map
Hordeum-Bins-7H73.5
Hordeum-Consensus2005-SNP-7H83.987
Hordeum-QTLConsensus-Abiotic-7H83.99
Hordeum-QTLConsensus-Agronomic-7H83.99
Hordeum-StMo-SNP-7H70.67
[ Show Nearby Loci ]
Map Data
Barley, Abiotic QTL Consensus
Barley, Agronomic QTL Consensus
Barley, BinMap 2005
Barley, Consensus 2005, SNP
Barley, Steptoe x Morex, SNP
Probe
HVM2
Reference
ReferenceRostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.