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GrainGenes Marker Report: WBE205

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Locus
WBE205
Type
CAPS
Chromosome
1H
Map
Hv-Consensus2006-Marcel-1H7.84
Hv-Integrated2009-1H10.1
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Map Data
Barley, Consensus 2006, Marcel
Barley, Integrated, Marcel 2009
Barley, Vada x SusPtrit
Probe
WBE205
Remark
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Vada x SusPtrit
Map position not determined on Vada x SusPtrit map
Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Vada x SusPtrit



Probe
WBE205
Locus
WBE205
Reference
ReferenceMarcel TC et al. (2007) A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues. Theoretical and Applied Genetics 114:487-500.
Remarks
Restriction Enzyme used for CAPS marker : Hin1I
Type
PCR
PCR primers
5' gagggtcgcaatcttcttttc 3'
5' agcacttggccttttatgaca 3'
Amplification Conditions
5 min at 94 deg C; 35 cycles with 30 sec at 94 deg C, 30 sec at 58 deg C, and 1 min at 72 deg C; and a final extension step of 7 min at 72 deg C
Source Species
Hordeum vulgare