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GrainGenes Marker Report: Xbcd1030

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Locus
Xbcd1030
Type
RFLP
Chromosome
5B
Map
Avena-Sorrells-B56.5
Wheat-Composite2004-5B93
[ Show Nearby Loci ]
Map Data
Diploid Oat, Cornell2
Wheat, Composite, 2004
Probe
BCD1030
Possible Orthologs
BCD1030
Xbcd1030-5B
bcd1030



Probe for Locus
BCD1030
Locus
Xbcd1030-5B
RI017F17
Xbcd1030
BCD1030
bcd1030
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Polymorphism
BCD1030 EcoRV
BCD1030 EcoRI
BCD1030 HindIII
BCD1030 XbaI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
1.3
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1030 SO autoradiogram
Data Source
Sorrells, Mark E.96.07