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GrainGenes Marker Report: Xbcd1094

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Locus
Xbcd1094
Type
RFLP
Map
Avena-Sorrells-G82.5
[ Show Nearby Loci ]
Map Data
Diploid Oat, Cornell2
Probe
BCD1094
Homology
AC079284_2e-value: 2.9(AC079284) hypothetical protein [Arabidopsis thaliana]
BAA96905.1e-value: 1e-18(AB019227) gene_id:MBM17.12~pir||T02538~strong similarity to unknown protein [Arabidopsis thaliana]



Probe for Locus
BCD1094
Locus
Xbcd1094
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438865
BE438888
BCD1094_WHE03F07.r1
BCD1094_WHE03F07.s1
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
1
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07