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GrainGenes Marker Report: Xbcd1150-1

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Locus
Xbcd1150-1
Type
RFLP
Chromosome Arm
1L
Map
Triticeae-Sorrells-180
[ Show Nearby Loci ]
Map Data
Triticeae consensus
Probe
BCD1150
Homology
S18135e-value: 0.57ryanodine receptor - pig (fragment)
NP_000602.1e-value: 3.9CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); CD59 antigen; Antigen identified by monoclonal antibody 16.3A5 [Homo sapiens] FACTOR) (MACIF) (MAC-INHIBITORY PROTEIN) (MAC-IP) (MEM43 ANTIGEN) (PROTECTIN) (MEMBRANE INHIBITOR OF REACTIVE LYSIS) (MIRL) (20 KDA HOMOLOGOUS RESTRICTION FACTOR) (HRF-20) (HRF20) (1F5 ANT>
Possible Orthologs
bcd1150
[ Show all 14 ]



Probe for Locus
BCD1150
Locus
Xbcd1150-1
[ Show all 19 ]
Synonym
SynonymHAR35_36339
HAR35_47249
HAR35_762
HAR35_763
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438620
BE438746
BCD1150_WHE1H0032
BCD1150_WHE1H0031
Polymorphism
BCD1150 EcoRI
BCD1150 EcoRV
BCD1150 DraI
BCD1150 HindIII
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
1.8
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1150 KO autoradiogram
BCD1150 SO autoradiogram
Data Source
Sorrells, Mark E.96.07