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GrainGenes Marker Report: Xbcd1262

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Locus
Xbcd1262
Type
RFLP
Chromosome
4B
4D
Chromosome Arm
4DL
Map
Ta-Physical-Gill-4ABD23
Ta-Physical-Gill-4D1.17
Tm-Dubcovsky2-4A0
Wheat-Composite2004-4B22
[ Show Nearby Loci ]
QTL with Locus Marker
QPm.ipk-4B
Breakpoint Interval
4DL-5/4DL-6
4L-0.10-0.35
Map Data
T. monococcum, DV92 x G3116
Wheat, Composite, 2004
Wheat, Physical
Species
Triticum aestivum
Probe
BCD1262
Homology
AAB41706.1e-value: 0.042(U73500) large T antigen [JC virus]
Reference
ReferenceBoyko EV et al. (1999) A high-density genetic linkage map of Aegilops tauschii, the D-genome progenitor of bread wheat. Theoretical and Applied Genetics 99:16-26.
Remark
In the population 'T. monococcum, DV92 x G3116,' this locus was completely linked to Xpsr327, Xpsr153, Xpsr920, Xabg484, Xbcd1006, XksuH8-4A, and Xcdo388-4A. Segregation data are listed under Xbcd327.
Possible Orthologs
BCD1262
Xbcd1262-4A
Xbcd1262-4B
Xbcd1262-4D
bcd1262



Probe for Locus
BCD1262
Locus
Xbcd1262-4B
[ Show all 6 ]
Synonym
SynonymHAR35_15305
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Bar.LambZAPII
Type
cDNA
Sequence
BE438882
BCD1262_WHE03G02.r1
Polymorphism
BCD1262 EcoRI
BCD1262 XbaI
Linkage Group
4L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1
PCR size
1
Clone Vector
pBluSK
pBluSK-
pGEM4
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1262 BROWS autoradiogram
BCD1262 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09