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GrainGenes Marker Report: Xbcd1285-4A

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Locus
Xbcd1285-4A
Type
RFLP
Map
Ta-Synthetic/Opata-GPW-4A55.8
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Map Data
Wheat, Synthetic x Opata, GPW
Probe
BCD1285
Possible Orthologs
Xbcd1285



Probe for Locus
BCD1285
Locus
Xbcd1285-4A
Xbcd1285
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
1.1
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07