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GrainGenes Marker Report: Xbcd131-3D

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Locus
Xbcd131-3D
Type
RFLP
Chromosome
3D
Chromosome Arm
3DL
Map
Ta-Gale-3D56.9
[ Show Nearby Loci ]
Map Data
Wheat, Gale
Probe
BCD131
Homology
A34690e-value: 0.74DNA-binding protein inhibitor Id - mouse (fragment)
T23581e-value: 5.1hypothetical protein K10G4.5 - Caenorhabditis elegans domain: PF01827 (Domain of unknown function), Score=188.0, E-value=4.9e-53, N=4 [Caenorhabditis elegans]
Possible Orthologs
bcd131
[ Show all 8 ]



Probe for Locus
BCD131
Locus
bcd131
[ Show all 11 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Bar.LambZAPII
Type
cDNA
Sequence
BE438613
[ Show all 6 ]
Polymorphism
BCD131 EcoRV
Linkage Group
[b 3L]
[b 3L]
3
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1
PCR size
1
Clone Vector
pBluSK
pBluSK-
BS-SK-
pBS
lambda_Zap
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
m13reverse 5'AACAGCTATGACCATG
m13forward 5'GTAAAACGACGGCCACT
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Lapitan, Nora
Image
BCD131 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
Graner, Andreas94.03.08
ATMI92.09
Kleinhofs, Andris96.05.08
Gill, Bikram S.92.09