Query (optional)   in Class  

GrainGenes Marker Report: Xbcd141-3A

[Submit comment/correction]

Locus
Xbcd141-3A
Type
RFLP
Chromosome
3A
Chromosome Arm
3AL
Map
3A, CNN x WI88.1
Tturgidum-JKxC2-3A92.7
[ Show Nearby Loci ]
QTL with Locus Marker
Qkps.unl-3A.2
QSpsm.sha-3A
Map Data
3A, CNN x WI
T. turgidum, JK x Cham1, 2
Probe
BCD141
Homology
AAF54122.1e-value: 5.4(AE003675) CG10272 gene product [Drosophila melanogaster]
T48615e-value: 1e-15hypothetical protein F18O22.210 - Arabidopsis thaliana
Possible Orthologs
bcd141
Xbcd141
Xbcd141-3B



Probe for Locus
BCD141
Locus
Xbcd141-3A
Xbcd141-3B
RI038D14
Xbcd141
bcd141
Synonym
SynonymHAR35_17539
HAR35_17540
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438875
BE438897
BCD141_WHE03G11.r1
BCD141_WHE03G11.s1
Polymorphism
BCD141 EcoRV
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
1
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD141 SO autoradiogram
Data Source
Sorrells, Mark E.96.07

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.