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GrainGenes Marker Report: Xbcd1495

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Locus
Xbcd1495
Type
RFLP
Chromosome
6B
Map
Avena-Sorrells-C40.2
Oat-KxO-1369
Wheat-Composite2004-6B119
[ Show Nearby Loci ]
Map Data
Diploid Oat, Cornell2
Oat, KxO
Wheat, Composite, 2004
Probe
BCD1495
Homology
AF346305_1e-value: 4e-47(AF346305) S-adenosyl-L-methionine synthetase [Elaeagnus umbellata]
METL_CATROe-value: 2e-60S-ADENOSYLMETHIONINE SYNTHETASE 2 (METHIONINE ADENOSYLTRANSFERASE 2) (ADOMET SYNTHETASE 2)
METL_CATROe-value: 2e-37S-ADENOSYLMETHIONINE SYNTHETASE 2 (METHIONINE ADENOSYLTRANSFERASE 2) (ADOMET SYNTHETASE 2)
METL_CATROe-value: 2e-49S-ADENOSYLMETHIONINE SYNTHETASE 2 (METHIONINE ADENOSYLTRANSFERASE 2) (ADOMET SYNTHETASE 2)
Image
BCD1495 KO autoradiogram
Possible Orthologs
bcd1495
[ Show all 8 ]



Probe for Locus
BCD1495
Locus
Xbcd1495-6B
[ Show all 9 ]
Related Probe
CDO1421
Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438821
[ Show all 8 ]
Polymorphism
BCD1495 EcoRI
BCD1495 EcoRV
BCD1495 DraI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
1.3
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1495 KO autoradiogram
BCD1495 SO autoradiogram
Data Source
Sorrells, Mark E.96.07