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GrainGenes Marker Report: Xbcd1686

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Locus
Xbcd1686
Type
RFLP
Map
Avena-Sorrells-C41.8
[ Show Nearby Loci ]
Map Data
Diploid Oat, Cornell2
Probe
BCD1686
Homology
AF361321_1e-value: 5e-14(AF361321) polygalacturonase [Pisum sativum]
AF361321_1e-value: 2e-21(AF361321) polygalacturonase [Pisum sativum]



Probe for Locus
BCD1686
Locus
Xbcd1686
Synonym
SynonymHAR35_47413
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438832
BE438856
BCD1686_WHE2A0111
BCD1686_WHE2A0112
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
0.5
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07