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GrainGenes Marker Report: Xbcd1716

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Locus
Xbcd1716
Type
RFLP
Chromosome
6B
Map
Oat-2003-OglexMAM17-5_150
Wheat-Composite2004-6B116
[ Show Nearby Loci ]
Map Data
Oat-2003-OglexMAM17-5
Wheat, Composite, 2004
Probe
BCD1716
Homology
AF159297_1e-value: 5e-05(AF159297) extensin-like protein [Zea mays]
AF159296_1e-value: 0.001(AF159296) extensin-like protein [Lycopersicon esculentum]
Possible Orthologs
bcd1716a
[ Show all 14 ]



Probe for Locus
BCD1716
Locus
Xbcd1716-6
[ Show all 16 ]
Synonym
Other nameBC1716
SynonymHAR35_14727
HAR35_14729
HAR35_14730
Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438818
BE438840
BCD1716_WHE2B0021
BCD1716_WHE2B0022
Polymorphism
BCD1716 XbaI
BCD1716 DraI
BCD1716 EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
0.6
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1716 KO autoradiogram
BCD1716 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
Sorrells, Mark E.94.10.31

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