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GrainGenes Marker Report: Xbcd1727

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Locus
Xbcd1727
Type
RFLP
Map
Avena-Sorrells-E78.7
[ Show Nearby Loci ]
Map Data
Diploid Oat, Cornell2
Probe
BCD1727
Homology
AC007591_2e-value: 1e-09(AC007591) Contains similarity to gb acid phosphatase alpha-2 (PAP2_a2) from Homo sapiens. ESTs gb|T88254 and gb|AA394650 come from this gene. [Arabidopsis thaliana]



Probe for Locus
BCD1727
Locus
Xbcd1727
Synonym
SynonymHAR35_291
HAR35_508
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438841
BCD1727_WHE2B0031
BCD1727_WHE2B0032
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
0.8
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07

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