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GrainGenes Marker Report: Xbcd180

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Locus
Xbcd180
Type
RFLP
Map
Oat-KxO-2297.4
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Map Data
Oat, KxO
Probe
BCD180
Possible Orthologs
bcd180
BCD180



Probe for Locus
BCD180
Locus
Xbcd180
bcd180
BCD180
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
0.8
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Image
BCD180 KO autoradiogram
Data Source
Sorrells, Mark E.96.07