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GrainGenes Marker Report: Xbcd1965

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Locus
Xbcd1965
Type
RFLP
Map
Avena-Sorrells-E20.7
Oat-KxO-2617.2
[ Show Nearby Loci ]
Map Data
Diploid Oat, Cornell2
Oat, KxO
Probe
BCD1965
Homology
C23703e-value: 3e-12ribulose-bisphosphate carboxylase activase (EC 6.3.4.-) A short form precursor - barley vulgare]
T06177e-value: 1e-72ribulose-bisphosphate carboxylase activase (EC 6.3.4.-) A1 - barley vulgare]
Image
BCD1965 KO autoradiogram
Possible Orthologs
bcd1965
BCD1965



Probe for Locus
BCD1965
Locus
Xbcd1965
bcd1965
BCD1965
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438827
BE438850
BCD1965_WHE2B0061
BCD1965_WHE2B0062
Polymorphism
BCD1965 EcoRI
BCD1965 EcoRV
BCD1965 DraI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
1.9
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1965 KO autoradiogram
Data Source
Sorrells, Mark E.96.07