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GrainGenes Marker Report: Xbcd1975

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Locus
Xbcd1975
Type
RFLP
Chromosome
4A
7D
Map
Wheat-Composite2004-4A152
Wheat-Composite2004-7D7
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
BCD1975
Possible Orthologs
BCD1975
Xbcd1975-4A
Xbcd1975-4A.1
Xbcd1975-4A.2
Xbcd1975-7D



Probe for Locus
BCD1975
Locus
Xbcd1975-7D
[ Show all 6 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Polymorphism
BCD1975 XbaI
BCD1975 EcoRI
BCD1975 EcoRV
BCD1975 HindIII
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
1
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Image
BCD1975 SO autoradiogram
Data Source
Sorrells, Mark E.96.07