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GrainGenes Marker Report: Xbcd204-5A

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Locus
Xbcd204-5A
Type
RFLP
Chromosome
5A
Chromosome Arm
5AL
Map
5AL, q deletions25
[ Show Nearby Loci ]
Breakpoint Interval
5AL-q37/5AL-q9
Map Data
5AL, CS q deletions, physical
Probe
BCD204
Possible Orthologs
BCD204
BCD204B
Xbcd204



Probe for Locus
BCD204
Locus
Xbcd204
BCD204
BCD204B
Xbcd204-5A
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Linkage Group
5L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.7
PCR size
0.7
Clone Vector
pBluSK
pBluSK-
lambda_Zap
pBS
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
m13reverse 5'AACAGCTATGACCATG
m13forward 5'GTAAAACGACGGCCACT
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07
ATMI92.09
Gill, Bikram S.92.09