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GrainGenes Marker Report: Xbcd266-2R

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Locus
Xbcd266-2R
Type
RFLP
Chromosome
2R
Map
Secale-N6xN2-2004-2R106.8
Secale-N7xN2-2004-2R154.7
Secale-N7xN6-2004-2R94
Secale-PxP-2004-2R113.8
[ Show Nearby Loci ]
Map Data
Rye, N6 x N2, 2004
Rye, N7 x N2, 2004
Rye, N7 x N6, 2004
Rye, P87 x P105, 2004
Species
Secale cereale
Probe
BCD266
Homology
AAD11455.2e-value: 1.3(U82549) sodium/potassium-transporting ATPase alpha-1 subunit [Oreochromis mossambicus]
AAD11455.2e-value: 2.1(U82549) sodium/potassium-transporting ATPase alpha-1 subunit [Oreochromis mossambicus]
Reference
ReferenceKhlestkina EK et al. (2004) Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags Theoretical and Applied Genetics 109:725-732.
Possible Orthologs
BCD266
[ Show all 8 ]



Probe for Locus
BCD266
Locus
Xbcd266-2
[ Show all 9 ]
Synonym
SynonymHAR35_15015
HAR35_15016
HAR35_33212
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Bar.LambZAPII
Type
cDNA
Sequence
BE438697
BE438789
BCD266_WHE1B0101
BCD266_WHE1B0102
Polymorphism
BCD266 EcoRI
Linkage Group
[b 2L]
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1.5
PCR size
1.5
Clone Vector
pBluSK
pBluSK-
BS-SK-
lambda_Zap
pBS
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
m13reverse 5'AACAGCTATGACCATG
m13forward 5'GTAAAACGACGGCCACT
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD266 BROWS autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09
Gill, Bikram S.92.09