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GrainGenes Marker Report: Xbcd292b

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Locus
Xbcd292b
Type
RFLP
Chromosome
2A
Map
Wheat-Composite2004-2A109
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
BCD292
Homology
BAB02849.1e-value: 4e-24(AB025634) gene_id:MSD21.18~unknown protein [Arabidopsis thaliana]
Possible Orthologs
BCD292
[ Show all 7 ]



Probe for Locus
BCD292
Locus
Xbcd292-2
[ Show all 8 ]
Synonym
SynonymHAR35_34125
HAR35_37250
HAR35_5434
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438736
BCD292_WHE1E0031
BCD292_WHE1E0032
Polymorphism
Durums BCD292 EcoRI
BCD292 EcoRI
Linkage Group
2L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.9
PCR size
0.9
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD292 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09