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GrainGenes Marker Report: Xbcd351

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Locus
Xbcd351
Type
RFLP
Chromosome
5B
5R
Chromosome Arm
5RL
Map
Avena-Sorrells-E105.8
[ Show all 6 ]
[ Show Nearby Loci ]
Breakpoint Interval
5DL-1/5DL-2
Map Data
Diploid Oat, Cornell2
[ Show all 6 ]
Probe
BCD351
Homology
BAA83564.1e-value: 1e-16(AP000399) ESTs C97429(C60159),D22427(C11106), AU078031(E31854),D15683(C1084) correspond to a region of the predicted gene.~hypothetical protein [Oryza sativa]
BAA83564.1e-value: 2e-11(AP000399) ESTs C97429(C60159),D22427(C11106), AU078031(E31854),D15683(C1084) correspond to a region of the predicted gene.~hypothetical protein [Oryza sativa]
Possible Orthologs
BCD351
[ Show all 18 ]



Probe for Locus
BCD351
Locus
BCD351C
[ Show all 31 ]
Synonym
SynonymHAR35_1862
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438985
BE439015
BCD351_WHE05B06.s1
Linkage Group
[b 1H, 2H]
Barley 5 (1H)
4
Barley 7 (5H)
2
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.9
PCR size
0.9
Clone Vector
pBluSK
pBluSK-
pBS
lambda_Zap
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
m13reverse 5'AACAGCTATGACCATG
m13forward 5'GTAAAACGACGGCCACT
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07
Graner, Andreas94.03.08
ATMI92.09
Kleinhofs, Andris96.05.08
Gill, Bikram S.92.09