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GrainGenes Marker Report: Xbcd385

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Locus
Xbcd385
Type
RFLP
Chromosome
4R
6R
7B
7D
Chromosome Arm
4RL
6RL
7DS
Map
Avena-Sorrells-E105.8
[ Show all 8 ]
[ Show Nearby Loci ]
Breakpoint Interval
7S-0.37-0.45
Map Data
Diploid Oat, Cornell2
[ Show all 7 ]
Species
Triticum aestivum
Probe
BCD385
Homology
BAB08647.1e-value: 5e-14(AB009048) frnE protein-like [Arabidopsis thaliana]
BAB08647.1e-value: 1e-08(AB009048) frnE protein-like [Arabidopsis thaliana]
Image
BCD385 KO autoradiogram
Reference
ReferenceBoyko EV et al. (1999) A high-density genetic linkage map of Aegilops tauschii, the D-genome progenitor of bread wheat. Theoretical and Applied Genetics 99:16-26.
Possible Orthologs
bcd385
Xbcd385-7B
BCD385
bcd385-7B



Probe for Locus
BCD385
Locus
Xbcd385-7B
bcd385
Xbcd385
BCD385
bcd385-7B
Synonym
SynonymHAR35_1722
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Bar.LambZAPII
Type
cDNA
Sequence
BE438610
BE438658
BE438725
BCD385_WHE1B0021
BCD385_WHE1B0022
Polymorphism
BCD385 EcoRI
BCD385 EcoRV
BCD385 DraI
BCD385 HindIII
BCD385 XbaI
Linkage Group
7LS, 7S
7S
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1
PCR size
1
Clone Vector
pBluSK
pBluSK-
BS-SK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD385 BROWS autoradiogram
BCD385 KO autoradiogram
BCD385 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09