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GrainGenes Marker Report: Xbcd508-5A

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Locus
Xbcd508-5A
Type
RFLP
Chromosome
5A
Chromosome Arm
5AL
Map
5A, DS 5AEurope50
5A, DS 5AIran85.2
Tm-Dubcovsky2-5A35.2
[ Show Nearby Loci ]
Map Data
5A, DS 5AEurope
5A, DS 5AIran
T. monococcum, DV92 x G3116
Probe
BCD508
Homology
T22388e-value: 4.6hypothetical protein F48F7.4 - Caenorhabditis elegans this gene~cDNA EST yk50c1.3 comes from this gene~cDNA EST yk50c1.5 comes from this gene~cDNA EST yk167b2.3 comes from this gene~cDNA EST yk167b2.5 comes from this gene~cDNA EST yk145e8.3>
Possible Orthologs
BCD508
[ Show all 17 ]



Probe for Locus
BCD508
Locus
Xbcd508-1
[ Show all 21 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438732
BCD508_WHE1D0031
BCD508_WHE1D0032
Polymorphism
BCD508 XbaI
Linkage Group
1L, 5L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1.4
PCR size
1.4
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD508 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09

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