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GrainGenes Marker Report: Xbcd512-2A

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Locus
Xbcd512-2A
Type
RFLP
Chromosome
2A
Chromosome Arm
2AL
Map
Ta-Gale-2A63.3
[ Show Nearby Loci ]
Map Data
Wheat, Gale
Probe
BCD512
Possible Orthologs
BCD512
[ Show all 10 ]



Probe for Locus
BCD512
Locus
BCD512
[ Show all 11 ]
Synonym
SynonymHAR35_1860
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438733
BCD512_WHE1D0042
BCD512_WHE1D0041
Linkage Group
[b 1L]
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.8
PCR size
0.8
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07
ATMI92.09