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GrainGenes Marker Report: Xbcd734

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Locus
Xbcd734
Type
RFLP
Chromosome
4D
Chromosome Arm
4DL
Map
Ta-Physical-Gill-4ABD44
Tm-Dubcovsky2-4A0.7
[ Show Nearby Loci ]
Breakpoint Interval
4L-0.41-0.46
Map Data
T. monococcum, DV92 x G3116
Wheat, Physical
Species
Triticum aestivum
Probe
BCD734
Homology
T45911e-value: 2e-10hypothetical protein F5K20.10 - Arabidopsis thaliana
T45911e-value: 7e-24hypothetical protein F5K20.10 - Arabidopsis thaliana
Reference
ReferenceBoyko EV et al. (1999) A high-density genetic linkage map of Aegilops tauschii, the D-genome progenitor of bread wheat. Theoretical and Applied Genetics 99:16-26.
Remark
In the population 'T. monococcum, DV92 x G3116,' this locus was completely linked to Xucd102(Esi13), Xttu1936(Hsp26.6a), Xmwg948, Xbcd1652, Ba, Xcdo1387, and Xmwg677. This locus and Xbcd1092 were mapped with identical restriction fragments, so the corresponding clones are assumed to be homologous. Segregation data are listed under Xucd102(Esi13).
Possible Orthologs
BCD734



Probe for Locus
BCD734
Locus
HD075J21
HD072K4
BCD734
Xbcd734
Synonym
SynonymHAR35_459
HAR35_510
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Bar.LambZAPII
Type
cDNA
Sequence
BE438737
[ Show all 6 ]
Polymorphism
BCD734 EcoRI
Linkage Group
4L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1.2
PCR size
1.2
Clone Vector
pBluSK
pBluSK-
BS-SK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD734 BROWS autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09