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GrainGenes Marker Report: Xbcd749

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Locus
Xbcd749
Type
RFLP
Chromosome
4B
Map
Wheat-Composite2004-4B23
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
BCD749
Possible Orthologs
Xbcd749-4B
Xbcd749-4D
bcd749



Probe for Locus
BCD749
Locus
Xbcd749-4B
Xbcd749-4D
Xbcd749
bcd749
Synonym
SynonymHAR35_17090
HAR35_43364
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438903
BCD749_WHE04A02.s1
Polymorphism
BCD749 DraI
BCD749 EcoRI
BCD749 EcoRV
BCD749 XbaI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
1.5
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD749 SO autoradiogram
Data Source
Sorrells, Mark E.96.07

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