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GrainGenes Marker Report: Xbcd87

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Locus
Xbcd87
Type
RFLP
Chromosome
5D
Map
Wheat-Composite2004-5D97
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
BCD87
Possible Orthologs
BCD87
[ Show all 10 ]



Probe for Locus
BCD87
Locus
Xbcd87-5D
[ Show all 11 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Linkage Group
5BL, 5DL, 7BS
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1.3
PCR size
1.3
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07
ATMI92.09