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GrainGenes Marker Report: Xbcd927

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Locus
Xbcd927
Type
RFLP
Chromosome
1R
Chromosome Arm
1RS
Map
Avena-Sorrells-C37
Rye-2009-Consensus-1R40.4
Secale-ExR-10
[ Show Nearby Loci ]
Map Data
Diploid Oat, Cornell2
Rye, ExR
Rye_2009_consensus
Probe
BCD927
Homology
E71602e-value: 0.69probable integral membrane protein PFB0950w - malaria parasite (Plasmodium falciparum) falciparum]
AC068667_7e-value: 1e-07(AC068667) plasma membrane associated protein, putative; 66162-66952 [Arabidopsis thaliana]
Possible Orthologs
BCD927



Probe for Locus
BCD927
Locus
Xbcd927
BCD927
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438829
BE438852
BCD927_WHE2H0061
BCD927_WHE2H0062
Linkage Group
3L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1.1
PCR size
1.1
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07
ATMI92.09