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GrainGenes Marker Report: Xbcd98-1R

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Locus
Xbcd98-1R
Type
RFLP
Chromosome
1R
Map
Secale-N6xN2-2004-1R27.5
Secale-N7xN2-2004-1R13.8
Secale-N7xN6-2004-1R6.9
[ Show Nearby Loci ]
Map Data
Rye, N6 x N2, 2004
Rye, N7 x N2, 2004
Rye, N7 x N6, 2004
Species
Secale cereale
Probe
BCD98
Homology
AAF35955.1e-value: 3e-42(AC024128) unknown protein [Arabidopsis thaliana]
AAF35955.1e-value: 2e-46(AC024128) unknown protein [Arabidopsis thaliana]
Reference
ReferenceKhlestkina EK et al. (2004) Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags Theoretical and Applied Genetics 109:725-732.
Possible Orthologs
BCD098
[ Show all 23 ]



Probe for Locus
BCD98
Locus
BCD98
[ Show all 29 ]
Related Probe
CDO99
Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Bar.LambZAPII
Type
cDNA
Sequence
BE438826
[ Show all 8 ]
Polymorphism
BCD98 EcoRI
Durums BCD98 EcoRI
BCD98 XbaI
Linkage Group
1S, 7S, 5H
1S,7S[b 5LS]
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
1.3
PCR size
1.3
Clone Vector
pBluSK
pBluSK-
BS-SK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD98 BROWS autoradiogram
BCD98 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09