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GrainGenes Marker Report: Xcdo1445

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Locus
Xcdo1445
Type
RFLP
Map
Avena-Sorrells-B11.6
[ Show Nearby Loci ]
Map Data
Diploid Oat, Cornell2
Probe
CDO1445
Possible Orthologs
cdo1445a
[ Show all 7 ]



Probe for Locus
CDO1445
Locus
Xcdo1445B
[ Show all 8 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Polymorphism
CDO1445 EcoRI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
PCR size
1.6
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Image
CDO1445 KO autoradiogram
Data Source
Sorrells, Mark E.96.07