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GrainGenes Marker Report: Xcdo196

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Locus
Xcdo196
Type
RFLP
Map
Avena-Sorrells-B77.4
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Map Data
Diploid Oat, Cornell2
Probe
CDO196
Possible Orthologs
CDO196



Probe for Locus
CDO196
Locus
CDO196
Xcdo196
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Polymorphism
CDO196 EcoRI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
PCR size
2
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07