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GrainGenes Marker Report: Xcdo270

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Locus
Xcdo270
Type
RFLP
Chromosome
6B
Map
Avena-Sorrells-A96.7
Wheat-Composite2004-6B91
[ Show Nearby Loci ]
Map Data
Diploid Oat, Cornell2
Wheat, Composite, 2004
Probe
CDO270
Homology
NP_070556.1e-value: 2.2galactosyltransferase [Archaeoglobus fulgidus] fulgidus
Possible Orthologs
CDO0270
[ Show all 17 ]



Probe for Locus
CDO270
Locus
Xcdo270-6
[ Show all 21 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Sequence
BE439259
CDO270_WHE04F02.s1
CDO270-5
CDO270-3
Polymorphism
CDO270 EcoRI
CDO270 EcoRV
CDO270 DraI
CDO270 XbaI
CDO270 HindIII
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
PCR size
1.5
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO270 KO autoradiogram
CDO270 SO autoradiogram
Data Source
Sorrells, Mark E.96.07