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GrainGenes Marker Report: Xcdo373-2D

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Locus
Xcdo373-2D
Type
RFLP
Chromosome
2D
2D Triticum aestivum
Chromosome Arm
2DL
Map
Ta-Augusta/Geneva-2D27.9
[ Show Nearby Loci ]
QTL with Locus Marker
QWss.cnl-2D
Map Data
2DL, Augusta x Geneva
Species
Triticum aestivum
Probe
CDO373
Homology
T05907e-value: 9e-09glycine hydroxymethyltransferase (EC 2.1.2.1) - barley (fragment) hydroxymethyltransferases [Hordeum vulgare]
Reference
ReferenceKhan AA et al. (2000) Identification of RFLP markers for resistance to wheat spindle streak mosaic bymovirus (WSSMV) disease Genome 43:477-482.
Remark
Along with Xbcd1095-2D, flanks the WSSMV resistance locus by 12.4 cM in the Geneva cultivar
Possible Orthologs
CDO373
Xcdo373
Xcdo373-2
Xcnl.CDO373



Probe for Locus
CDO373
Locus
Xcdo373-2
CDO373
Xcdo373
Xcnl.CDO373
Xcdo373-2D
Synonym
SynonymHAR35_46573
HAR35_47971
HAR35_605
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
OatUni-ZAPXR
Type
cDNA
Sequence
BE439108
CDO373_WHE2E0061
CDO373_WHE2E0062
Polymorphism
CDO373 EcoRI
Durums CDO373 EcoRI
CDO373 EcoRV
CDO373 DraI
Linkage Group
[b 2L]
2
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
0.9
PCR size
0.9
Clone Vector
pBluSK
BS-SK-
pBS
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO373 BROWS autoradiogram
CDO373 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09
Kleinhofs, Andris96.05.08