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GrainGenes Marker Report: Xcdo465

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Locus
Xcdo465
Type
RFLP
Map
Avena-Sorrells-E48.6
Tm-Dubcovsky2-5A66.4
[ Show Nearby Loci ]
Map Data
Diploid Oat, Cornell2
T. monococcum, DV92 x G3116
Probe
CDO465
Homology
T04414e-value: 3e-20probable glucan 1,3-beta-glucosidase (EC 3.2.1.58) ExoII - barley vulgare]
Possible Orthologs
CDO465
[ Show all 7 ]



Probe for Locus
CDO465
Locus
CDO465
[ Show all 12 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
BE439135
CDO465_WHE2G0092
CDO465_WHE2G0091
Linkage Group
[b 4L]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.8
PCR size
1.8
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07
ATMI92.09