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GrainGenes Marker Report: Xcdo475

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Locus
Xcdo475
Type
RFLP
Chromosome
7D
Chromosome Arm
7DS
7HS
Map
Hbulbosum-PB1xPB11-731
Ta-Physical-Gill-7ABD-84
Tm-Dubcovsky2-7A-33.5
[ Show Nearby Loci ]
Breakpoint Interval
7S-0.82-0.86
Map Data
H. bulbosum, PB1 x PB11
T. monococcum, DV92 x G3116
Wheat, Physical
Species
Triticum aestivum
Probe
CDO475
Reference
ReferenceBoyko EV et al. (1999) A high-density genetic linkage map of Aegilops tauschii, the D-genome progenitor of bread wheat. Theoretical and Applied Genetics 99:16-26.
Possible Orthologs
CDO475
[ Show all 12 ]



Probe for Locus
CDO475
Locus
CDO475
[ Show all 13 ]
External Databases
DDBJ
EMBL
GenBank
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
OatUni-ZAPXR
Type
cDNA
STS
PCR primers
GAC ACA TTG ACC GCA TCT TA
CCT TCA CCT CGC TCC CTA CC
Sequence
AA231764
AA231884
CDO475
Polymorphism
CDO475 EcoRI
CDO475 EcoRV
Linkage Group
7S [b 1S]
7S [b 1S]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks (oat)
Source Tissue
etiolated leaf
Insert Size
1.8
PCR size
1.8
Clone Vector
pBluSK
BS-SK-
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Blake, Tom
Image
CDO475 BROWS autoradiogram
CDO475 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
Blake, Tom95.08.24
ATMI92.09

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