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GrainGenes Marker Report: Xcdo539

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Locus
Xcdo539
Type
RFLP
Map
Avena-Sorrells-B30.4
Oat-2003-OglexMAM17-5_663
[ Show Nearby Loci ]
Map Data
Diploid Oat, Cornell2
Oat-2003-OglexMAM17-5
Probe
CDO539
Homology
BAB04949.1e-value: 1.2(AP001511) BH1230~unknown conserved protein in B. subtilis [Bacillus halodurans]
CAB51836.1e-value: 0.008(AJ243961) Putitive Ser/Thr protein kinase [Oryza sativa]
Possible Orthologs
CDO539a
[ Show all 8 ]



Probe for Locus
CDO539
Locus
CDO539a
[ Show all 9 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Sequence
BE439074
[ Show all 6 ]
Polymorphism
CDO539 EcoRI
Source Species
Avena sativa
Source Germplasm
Brooks (oat)
Source Tissue
etiolated leaf
PCR size
1.6
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO539 KO autoradiogram
Data Source
Sorrells, Mark E.96.07

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