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GrainGenes Marker Report: Xcdo57-7A

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Locus
Xcdo57-7A
Type
RFLP
Map
Tm-Dubcovsky2-7A-0.7
[ Show Nearby Loci ]
Map Data
T. monococcum, DV92 x G3116
Probe
CDO57
Possible Orthologs
CDO057
[ Show all 29 ]



Probe for Locus
CDO57
Locus
Xcdo57-2
[ Show all 33 ]
Related Probe
BCD981
[ Show all 6 ]
Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
BQ548073
BQ548072
CDO57-5
CDO57-3
Polymorphism
CDO57 EcoRV
CDO57 EcoRI
CDO57 XbaI
Linkage Group
7L,5L [b 2S]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.6
PCR size
1.6
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO57 KO autoradiogram
CDO57 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09