Query (optional)   in Class  

GrainGenes Marker Report: Xcdo57A

[Submit comment/correction]

Locus
Xcdo57A
Type
RFLP
Chromosome
5A
Map
Oat-2001-KxO_25-3237.5
[ Show all 8 ]
[ Show Nearby Loci ]
QTL with Locus Marker
Days to heading, KxO-32
[ Show all 6 ]
Map Data
Oat, KxO
[ Show all 8 ]
Probe
CDO57
Image
CDO57 KO autoradiogram
Reference
ReferenceJin H et al. (2000) Combined AFLP and RFLP mapping in two hexaploid oat recombinant inbred populations Genome 94-101.
Possible Orthologs
CDO057
[ Show all 29 ]



Probe for Locus
CDO57
Locus
Xcdo57-2
[ Show all 33 ]
Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
BQ548073
BQ548072
CDO57-5
CDO57-3
Polymorphism
CDO57 EcoRV
CDO57 EcoRI
CDO57 XbaI
Linkage Group
7L,5L [b 2S]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks (oat)
Source Tissue
etiolated leaf
Insert Size
1.6
PCR size
1.6
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO57 KO autoradiogram
CDO57 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.