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GrainGenes Marker Report: Xcdo669

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Locus
Xcdo669
Type
RFLP
Chromosome
4D
Map
Avena-Sorrells-E37.1
Tm-Dubcovsky2-4A-25.9
Wheat-Composite2004-4D49
[ Show Nearby Loci ]
Map Data
Diploid Oat, Cornell2
T. monococcum, DV92 x G3116
Wheat, Composite, 2004
Probe
CDO669
Homology
AF361799_1e-value: 1e-22(AF361799) At1g27150/T7N9_21 [Arabidopsis thaliana]
Possible Orthologs
CDO669
[ Show all 14 ]



Probe for Locus
CDO669
Locus
CDO669C
[ Show all 15 ]
Synonym
SynonymHAR35_5138
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
OatUni-ZAPXR
Type
cDNA
Sequence
BE439240
CDO669_WHE03A11.r1
CDO669_WHE03A11.s1
Polymorphism
CDO669 EcoRI
Durums CDO669 EcoRI
CDO669 EcoRV
CDO669 DraI
Linkage Group
4S [b 4S]
4S [b 4S]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.3
PCR size
1.3
Clone Vector
pBluSK
BS-SK-
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO669 BROWS autoradiogram
CDO669 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09