Query (optional)   in Class  

GrainGenes Marker Report: Xcfa2076-3A

[Submit comment/correction]

Locus
Xcfa2076-3A
Type
Microsatellite
Chromosome
3A
Map
Ta-Courtot/CS-GPW-3A204.7
Ta-SSR-2004-3A97.7
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Wheat, Courtot x Chinese Spring, GPW
Probe
CFA2076
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfa2076-5A
cfa2076-3A
cfa2076



Probe for Locus
CFA2076
Locus
Xcfa2076-3A
[ Show all 6 ]
Reference
ReferenceSourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (TG)18
Regularity of motif is imperfect
Type
PCR
SSR
PCR primers
Forward 5' CGAAAAACCATGATCGACAG 3'
Reverse 5' ACCTGTCCAGCTAGCCTCCA 3'
SSR size
172
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
3D
Source Species
Triticum urartu
Data Source
Sourdille, Pierre

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.