GrainGenes Marker Report: Xcfa2170-3B

GrainGenes Marker Report: Xcfa2170-3B

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Locus

Type

Microsatellite

Chromosome

3B

Chromosome Arm

3BL

Map

Chinese_Spring_Deletion_SSR_3B	0.815
[ Show all 6 ]

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Breakpoint Interval

Map Data

Wheat, Arina x Forno

[ Show all 6 ]

Species

Triticum aestivum

Probe

Reference

Reference

Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.

Reference

Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.

Reference

Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.

Possible Orthologs

[ Show all 8 ]

Probe for Locus

Locus

[ Show all 10 ]

Reference

Reference

Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.

General Remarks

Quality Score = B

Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)

Amplifies motif: (GA)31

Regularity of motif is imperfect

Type

PCR

SSR

PCR primers

Forward 5' TGGCAAGTAACATGAACGGA 3'
Reverse 5' ATGTCATTCATGTTGCCCCT 3'

SSR size

199

Amplification Conditions

5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees

Linkage Group

3A/3B

Source Species

Triticum urartu

Data Source

Sourdille, Pierre


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