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GrainGenes Marker Report: Xcfa2170a

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Locus
Xcfa2170a
Type
Microsatellite
Chromosome
1D
Map
Wheat-Composite2004-1D2
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Map Data
Wheat, Composite, 2004
Probe
CFA2170
Possible Orthologs
cfa2170
[ Show all 8 ]



Probe for Locus
CFA2170
Locus
Xcfa2170-3B
[ Show all 10 ]
Reference
ReferenceSourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (GA)31
Regularity of motif is imperfect
Type
PCR
SSR
PCR primers
Forward 5' TGGCAAGTAACATGAACGGA 3'
Reverse 5' ATGTCATTCATGTTGCCCCT 3'
SSR size
199
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
3A/3B
Source Species
Triticum urartu
Data Source
Sourdille, Pierre

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