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GrainGenes Marker Report: Xcfa2292-1B

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Locus
Xcfa2292-1B
Type
Microsatellite
Chromosome
1B
Chromosome Arm
1BL
Map
Chinese_Spring_Deletion_SSR_1B0.925
Ta-Courtot/CS-GPW-1B155.4
Triticum-AxF-1B177
[ Show Nearby Loci ]
Breakpoint Interval
1BL3-0.83-1.00
1BL3-0.85-1.00
Map Data
Wheat, Arina x Forno
Wheat, Courtot x Chinese Spring, GPW
Wheat, Physical, SSR
Species
Triticum aestivum
Probe
CFA2292
Reference
ReferencePaillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
Possible Orthologs
Xcfa2292



Probe for Locus
CFA2292
Locus
Xcfa2292-1B
Xcfa2292
Reference
ReferenceSourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = A
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (CA)20
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' GGACCGTTTATCCGTAAGCA 3'
Reverse 5' GCCTATGCTGCTGATCCATT 3'
SSR size
192
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
1B
Source Species
Triticum urartu
Data Source
Sourdille, Pierre

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