Query (optional)   in Class  

GrainGenes Marker Report: Xcfd189-5D

[Submit comment/correction]

Locus
Xcfd189-5D
Type
Microsatellite
Chromosome
5D
Map
Chinese_Spring_Deletion_SSR_5D-0.315
Ta-SSR-2004-5D14.6
Ta-Synthetic/Opata-GPW-5D14.4
[ Show Nearby Loci ]
Breakpoint Interval
C-5DS1-0.63
Map Data
Wheat, Consensus SSR, 2004
Wheat, Physical, SSR
Wheat, Synthetic x Opata, GPW
Probe
CFD189
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfd189
cfd189



Probe for Locus
CFD189
Locus
Xcfd189-5D
Xcfd189
cfd189
Reference
ReferenceGuyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = B
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (GT)28
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' GCTAAAGCCACATAGGACGG 3'
Reverse 5' GCACAAGATTTTGCAAGGCT 3'
SSR size
280
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
5D
Source Species
Aegilops tauschii
Data Source
Sourdille, Pierre

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.