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GrainGenes Marker Report: Xcfd267-2D

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Locus
Xcfd267-2D
Type
Microsatellite
Map
Ta-Synthetic/Opata-GPW-2D216.3
[ Show Nearby Loci ]
Map Data
Wheat, Synthetic x Opata, GPW
Probe
CFD267
Possible Orthologs
Xcfd267-2A
Xcfd267-2B
Xcfd267b



Probe for Locus
CFD267
Locus
Xcfd267-2A
Xcfd267-2B
Xcfd267-2D
Xcfd267b
Reference
ReferenceGuyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = D
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (GGC)6
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' GTGCGTCGTGTAGCAGCTC 3'
Reverse 5' CTCTCTGTCGTCCAGGTCGT 3'
SSR size
280
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
2A/2B/2D
Source Species
Aegilops tauschii
Data Source
Sourdille, Pierre