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GrainGenes Marker Report: Xcfd59b

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Locus
Xcfd59b
Type
Microsatellite
Chromosome
1B
Map
Wheat-Composite2004-1B28
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
CFD59
Possible Orthologs
Xcfd59-1A
[ Show all 13 ]



Probe for Locus
CFD59
Locus
Xcfd59-1B
[ Show all 14 ]
Reference
ReferenceGuyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = B
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (GC)6(GA)23
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' TCACCTGGAAAATGGTCACA 3'
Reverse 5' AAGAAGGCTAGGGTTCAGGC 3'
SSR size
296
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
1D/1B/6B/1D
Source Species
Aegilops tauschii
Data Source
Sourdille, Pierre

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