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GrainGenes Marker Report: Xcfd77a

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Locus
Xcfd77a
Type
Microsatellite
Chromosome
2D
Map
Wheat-Composite2004-2D5
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Map Data
Wheat, Composite, 2004
Probe
CFD77
Possible Orthologs
Xcfd77-2D



Probe for Locus
CFD77
Locus
Xcfd77-2D
Xcfd77a
Reference
ReferenceGuyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = C
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (GGC)6
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' CTGCTTCAGGGATTGGAGAG 3'
Reverse 5' GTTTCCTGGGCTAAACCACA 3'
SSR size
209
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
2D
Source Species
Aegilops tauschii
Data Source
Sourdille, Pierre

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