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GrainGenes Marker Report: Xgwm319

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Locus
Xgwm319
Type
Microsatellite
Chromosome
2B
Map
Wheat, UC1110 x PI610750 2B85.1
Wheat-Composite2004-2B75
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Wheat, UC1110 x PI610750
Probe
WMS319
Reference
ReferenceLowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs
Xgwm319-2B
Xgwm319-7A
gwm319
gwm319-2B



Probe for Locus
WMS319
Locus
Xgwm319-2B
[ Show all 6 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)11(N)23(CT)6
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' GGTTGCTGTACAAGTGTTCACG 3'
5' CGGGTGCTGTGTGTAATGAC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS319
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
Data Source
Roeder, Marion S.98.09.15