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GrainGenes Marker Report: Xgwm515-2A

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Locus
Xgwm515-2A
Type
Microsatellite
Chromosome
2A
Map
Chinese_Spring_Deletion_SSR_2A-0.39
[ Show all 10 ]
[ Show Nearby Loci ]
Breakpoint Interval
C-2AS5-0.78
Map Data
Durum wheat, Kofa x UC1113
[ Show all 10 ]
Probe
WMS515
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source
Roeder, Marion S.98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-2A, the intervals most likely to contain locus are Xbcd152 - Xgwm71.2 or Xgwm71.2 - Xbcd543
Possible Orthologs
Xgwm515
[ Show all 7 ]



Probe for Locus
WMS515
Locus
Xgwm515-2A
[ Show all 8 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GT)17(TCAT)(GT)6
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' AACACAATGGCAAATGCAGA 3'
5' CCTTCCTAGTAAGTGTGCCTCA 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS515
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
Data Source
Roeder, Marion S.98.09.15