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GrainGenes Marker Report: Xgwm666

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Locus
Xgwm666
Type
Microsatellite
Chromosome
1A
5A
Map
Wheat-Composite2004-1A98
Wheat-Composite2004-5A68
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMS666
Possible Orthologs
Xgwm666-1A
[ Show all 17 ]



Probe for Locus
WMS666
Locus
Xgwm666-3A
[ Show all 18 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CA)13
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' GCACCCACATCTTCGACC 3'
5' TGCTGCTGGTCTCTGTGC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS666
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
Data Source
Roeder, Marion S.98.09.15